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Part:BBa_J23074:Design

Designed by: Samantha Liang   Group: iGEM06_Berkeley   (2006-08-25)


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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1665
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 140
    Illegal NgoMIV site found at 266
    Illegal NgoMIV site found at 449
    Illegal NgoMIV site found at 710
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 967
    Illegal NgoMIV site found at 1463
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1771


Design Notes

pSB1A2-J23038 has a really short sequence between XbaI and SpeI so okay to PCR because miniprep was messy.


Source

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Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 )

Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229)

Product is pSB1AK3-J23074.

References